牛生乳由来IgG高含有濃縮乳清タンパクによる腸管出血性大腸菌O157:H7、腸炎菌、抗酸菌の感染防御

Food Science & Nutrition 2019 Jul 17;7(8):2726-2730

Enriched bovine IgG fraction prevents infections with Enterohaemorrhagic Escherichia coli O157:H7, Salmonella enterica serovar Enteritidis, and Mycobacterium avium

Keiji Funatogawa¹, Tatsuya Tada², Kyoko Kuwahara-Arai², Teruo Kirikae², Masao Takahashi³

1. Tochigi Prefectural Institute of Public Health and Environmental Science Utsunomiya Japan.
2. Department of Microbiology Juntendo University School of Medicine Tokyo Japan.
3. Aotearoa Co. Tokyo Japan.

A bovine IgG-enriched whey fraction contains antibodies against various bacterial antigens. We investigated the protective effects of a bovine whey fraction preparation against infections with Enterohaemorrhagic Escherichia coli O157:H7, Salmonella enterica serovar Enteritidis, and Mycobacterium avium in mouse models. After infection with these pathogens, the IgG-enriched fraction or skim milk was given ad libitum at a 5% solution instead of water. The mice given the IgG-enriched fraction were significantly resistant to orally challenged EHEC O157:H7 (LD50: 4.0 × 105 CFU/mouse) infections compared with the mice given skim milk (LD50: <1.5 × 102 CFU/mouse). The mice given the IgG-enriched fraction were also significantly resistant to orally challenged S. Enteritidis (LD50: 5.0 × 106 CFU/mouse) infections compared with the mice given skim milk (LD50: <2.5 × 101 CFU/mouse). When the mice were nasally infected with M. avium, the numbers of the bacteria in lungs of mice given the IgG-enriched fraction were significantly lower than those given skim milk 2 and 3 weeks after infection. These results strongly indicate that oral administration of the bovine IgG-enriched whey fraction protects mice against food-borne infection and also that it partially protects mice against respiratory tract infection.